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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4088R |
| 英文名称 | SLC27A5 Rabbit pAb |
| 中文名称 | 脂肪酸转运蛋白5抗体 |
| 英文别名 | ACSB; ACSVL6; BA CoA ligase; BACS; BAL; Bile acid CoA ligase; Bile acyl CoA synthetase; Cholate CoA ligase; FACVL3; FATP 5; FATP5; Fatty acid Coenzyme A ligase very long chain 3; Fatty acid transport protein 5; Solute carrier family 27(fatty acid transporter) member 5; Solute carrier family 27 member 5; Very long chain acyl CoA synthetase homolog 2; Very long chain acyl CoA synthetase related protein; VLACS related; VLACSR; VLCS H2; VLCSH2; S27A5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SLC27A5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 75 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Synthesis Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation Signal Transduction > Metabolism > Lipid metabolism |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| 组织特异性 | Predominantly expressed in liver. |
| 相似性 | Belongs to the ATP-dependent AMP-binding enzyme family. |
| 功能 | Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SLC27A5 is an isozyme of very long chain acyl CoA synthetase (VLCS). It is an acyl CoA synthetase involved in bile acid metabolism and is proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. It seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, it also activates 3 alpha,7 alpha,12 alpha trihydroxy 5 beta cholestanate (THCA), the C27 precursor of cholic acid derived from the de novo synthesis from cholesterol. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 5.201 }}] {Wang X et al. MicroRNA-20a-5p Ameliorates Non-alcoholic Fatty Liver Disease via Inhibiting the Expression of CD36Front Cell Dev Biol.2020 Dec 3;8:596329.} {WB} {Human}
[IF={{ 2.25 }}] {Han, Ying, et al. "Basis of aggravated hepatic lipid metabolism by chronic stress in high-fat diet-fed rat." Endocrine: 1-10.} {WB} {="Rat"}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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