Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Annexin A10 antibody (bs-5127R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 5,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-5127R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5127R |
| 英文名称 | Annexin A10 Rabbit pAb |
| 中文名称 | 膜粘连蛋白10抗体 |
| 英文别名 | annexin 14; Annexin A10; Annexin-10; Annexin-14; ANX 14; ANX10_HUMAN; ANX14; ANXA 10; ANXA10. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Annexin A10 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Annexins Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins |
| 相似性 | Belongs to the annexin family.
Contains 4 annexin repeats. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
A549(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Annexin A10 (bs-5127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
A549(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Annexin A10 (bs-5127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 2.11 }}] {Lu, Ying, et al. "Annexin A10 is involved in the development and maintenance of neuropathic pain in mice." Neuroscience Letters (2016).} {WB,IHC} {="Mouse"}
技术资料暂无技术资料 索取技术资料
文献支持
Annexin A10 Rabbit pAb(bs-5127R)-50ul/100ul/200ul
￥1180 - 2800
