Blank control:U937.
Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-2680R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2680R |
| 英文名称 | NFAM1 Rabbit pAb |
| 中文名称 | NFAM1抗体 |
| 英文别名 | NFAT activation molecule 1 precursor; NFAT activation molecule 1 precursor; Calcinerin/NFAT activating ITAM containing protein; CNAIP; NFAT activation molecule 1; Calcineurin/NFAT-activating ITAM-containing protein; NFAT-activating protein with ITAM motif 1; NFAM1_HUMAN. |
| 产品应用 | Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NFAM1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 30 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > NFATs Immunology > Innate Immunity > Cytokines > Interleukins Immunology > Innate Immunity > Cytokines > TNF Superfamily Signal Transduction > Signaling Pathway > Nuclear Signaling > NFATS |
| 亚基 | Interacts with ZAP70 and SYK. No direct interaction with the B-cell antigen receptor (BCR). |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Partially recruited to lipid rafts upon BCR stimulation. |
| 组织特异性 | Highly expressed in neutrophils, primary monocytes, mast cells, monocytic cell lines and lymphocytes. Also expressed in spleen B and T cells, and lung. Expressed at low level in non-immune tissue. |
| 翻译后修饰 | N-glycosylated. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 1 ITAM domain. |
| 功能 | May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters. May be involved in the regulation of B-cell, but not T-cell, development. Overexpression activates downstream effectors without ligand binding or antibody cross-linking. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | NFAM1 activates cytokine gene promoters such as the IL13 and TNF-alpha promoters. It contains an immunoreceptor tyrosine-based activation motif (ITAM) and is thought to regulate the signaling and development of B-cells. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 6.543 }}] {Zhu Jianbing. et al. Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1. OXID MED CELL LONGEV. 2022;2022:8392313} {WB} {Human}
[IF={{ 4.14 }}] {Sambandam et al. NFAM1 signaling enhances osteoclast formation and bone resorption activity in Paget's disease of bone. (2017) Bone. 101:236-244} {WB} {Rat}
相关实验
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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NFAM1 Rabbit pAb(bs-2680R)-50ul/100ul/200ul
￥1180 - 2800
