Tissue/cell: Human Cervical Cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Spindlin 1 Polyclonal Antibody, Unconjugated(bs-0724R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0724R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0724R |
| 英文名称 | Spindlin 1 Rabbit pAb |
| 中文名称 | 卵巢癌相关蛋白抗体 |
| 英文别名 | Spindlin-like protein 2; SPIN-2; DXF34; OCR; Ovarian cancer-related protein; SPIN; Spin1; SPIN1_HUMAN; Spindlin-1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Spindin 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell division Cancer > Cell Death > Apoptosis > Nucleus Cell Biology > Apoptosis > Nucleus Cell Biology > Cell Cycle > Cell Division > Spindle Developmental Biology > Reproduction > Germ cell markers Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding |
| 亚基 | Homodimer; may form higher-order oligomers. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Phosphorylated during oocyte meiotic maturation. |
| 相似性 | Belongs to the SPIN/STSY family. |
| 功能 | May play a role in cell-cycle regulation during the transition from gamete to embryo (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Spindlin 1 (Spindlin-like protein 2; SPIN-2 ) protein May play a role in cell-cycle regulation during the transition from gamete to embryo. Subcellular location in the Nucleus. Belongs to the SPIN/STSY family. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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Spindlin 1 Rabbit pAb(bs-0724R)-50ul/100ul/200ul
￥1180 - 2800
