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- 2025年10月24日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10999R |
| 英文名称 | phospho-PLB (Ser16+Thr17) Rabbit pAb |
| 中文名称 | 磷酸化心脏磷蛋白(Ser16+Thr17)抗体 |
| 英文别名 | phospholamban(phospho S16+T17); p-phospholamban(phospho S16+T17); phospho-phospholamban(Ser16/Thr17); phospholamban(phospho Ser16+Thr17); p-PLB(S16+T17); Cardiac phospholamban; CMD1P; PLB; PLN; PPLA_HUMAN. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human PLB around the phosphorylation site of Ser16+Thr17 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 6 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Cardiovascular > Heart > Contractility > Inotropics Cardiovascular > Vasculature > Vasodilation Signal Transduction > Metabolism > Plasma Membrane > ATPases Signal Transduction > Metabolism > Plasma Membrane > Channels Signal Transduction > Signaling Pathway > Calcium Signaling Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Channels Tags & Cell Markers > Cell Type Markers > Other Cell Types |
| 亚基 | Homopentamer. Interacts with HAX1. |
| 亚细胞定位 | Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum. |
| 组织特异性 | Heart. |
| 翻译后修饰 | Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. |
| 相似性 | Belongs to the phospholamban family. |
| 功能 | Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Sarco(endo)plasmic-reticulum (SER) regulatory protein, Phospholamban (PLB), is a small, plasma membrane-associated phospho-protein found in the SER of cardiac, smooth and slow-twitch muscle. Believed to assemble into a pentamer, PLB regulates cardiac contractility and Ca2+ affinity for cardiac SER Ca2+ ATPase (SERCA2a). Non-phosphorylated PLB associates with SERCA2a, and inhibits Ca2+ reuptake into the SER. PLB activation occurs when key Serine/Threonine residues in PLB (Ser-10, Ser-16, Thr-17) are phosphorylated by numerous effectors, which include PKC, PKA, PKG, and CaM kinase. Phosphorylation of PLB causes dissociation from SERCA2a and a subsequent increase in the rate of Ca2+ reuptake into the SER, which accelerates ventricular relaxation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
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4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
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