- ￥1180 - 2800
- Bioss
- bs-13020R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
FHOD1 Rabbit pAb, BF350 conjugated(bs-13158R-BF350)-100ul
￥2980
CCL21 Rabbit pAb(bs-1666R)-50ul/100ul/200ul
￥1180phospho-AKT1 (Tyr474) Rabbit pAb, BF680 conjugated(bs-10133R-BF680)-100ul
￥2980
MTOR Rabbit pAb, APC conjugated(bs-1992R-APC)-100ul
￥2980phospho-Histone H3 (Thr32) Rabbit pAb(bs-17441R)-50ul/100ul/200ul
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13020R |
| 英文名称 | DNA Polymerase lambda Rabbit pAb |
| 中文名称 | DNA聚合酶λ/DNA pol λ抗体 |
| 英文别名 | BETA N; BETAN; DNA directed DNA polymerase lambda; DNA polymerase beta 2; DNA polymerase beta-2; DNA polymerase kappa; DNA polymerase kappa DNA polymerase beta N; DNA polymerase lambda; DNA polymerase lamda2; DPOLL_HUMAN; EC 2.7.7.7, EC 4.2.99.; FLJ46002; OTTHUMP00000020321; OTTHUMP00000020323; OTTHUMP00000059179; Pol beta2; POL KAPPA; Pol Lambda; POLKAPPA; POLL; Polymerase DNA directed lambda. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Chicken, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNA Polymerase lambda |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 63 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds PCNA. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in a number of tissues. Abundant in testis. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain. |
| 功能 | Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | DNA polymerase lambda (pol lambda), also designated DNA polymerase lambda or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol lambda is a member of the DNA polymerase type-X family. Pol lambda extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ?may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol lambda and thus influences the ability of pol ?to synthesize DNA. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Recombineering/Lambda red-mediated gene replacement
Electroprate linear DNA into electrocompetent cells Grow at 37°C on chloramphenicol plates PCR verify the deletion with oligos C and D (see below for design) Detailed procedure Day 0: Start overnight culture
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
fine.) Alkaline Phosphatase Buffer 100 mM Tris-HCl, pH 9.5 100 mM NaCl 5 mM MgCl2 上一篇：BENTON DAVIS BLOTS 下一篇：SCREENING A LAMBDA BACTERIOPHAGE GENOMIC LIBRARY WITH A DNA PROBE
polymerase enzyme. The primers match some sequence in the target DNA. The solution is rapidly heated to DNA denaturing temperatures (~95°C) and cooled to a temperature where the polymerase can function. Each thermal cycle generates copies of the sequence
技术资料暂无技术资料 索取技术资料
