Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MAP3K9+MAP3K10(Thr312 + Thr266) (bs-6779R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121/105 kD
Observed band size: 121 kD
- ￥1180 - 2800
- Bioss
- bs-6779R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6779R |
| 英文名称 | phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Rabbit pAb |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶3K9抗体 |
| 英文别名 | MAP3K9+MAP3K10(phospho T312+T266); MAP3K9+MAP3K10(phospho T312+T266); phospho-MAP3K9(Thr312)/MAP3K10( Thr266); p-MAP3K9; M3K9_HUMAN; M3K10_HUMAN; MAPKKK9; MEKK10; MEKK9; Mitogen activated protein kinase kinase kinase 10; Mitogen activated protein kinase kinase kinase 9; Mixed lineage kinase 1(tyr and ser/thr specificity); Mixed lineage kinase 2; MKN28 derived nonreceptor_type serine/threonine kinase; MKN28 kinase; MLK1; MLK2; MST; PRKE1; Protein kinase MST. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human MAP3K9/MAP3K10 around the phosphorylation site of Thr312/Thr266 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 121/105 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Apoptosis > Intracellular > Kinases Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway Signal Transduction > Protein Phosphorylation > Tyrosine Kinases |
| 亚基 | Homodimer. |
| 组织特异性 | Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. |
| 翻译后修饰 | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308. |
| 相似性 | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain. |
| 功能 | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MAP3K9+MAP3K10(Thr312 + Thr266) (bs-6779R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121/105 kD
Observed band size: 121 kD
A431(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MAP3K9+MAP3K10(Thr312 + Thr266) (bs-6779R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121/105 kD
Observed band size: 121 kD
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文献和实验该产品被引用文献
[IF={{ 3.197 }}] {Menghua Li. et al. Murine cytomegalovirus employs the mixed lineage kinases family to regulate the spiral ganglion neuron cell death and hearing loss. NEUROSCI LETT. 2022 Nov;:136990} {IF,WB} {Mouse}
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phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Rabbit pAb(bs-6779R)-50ul/100ul/200ul
￥1180 - 2800
