Sample:
HepG2(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- HEPACAM (bs-5840R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 62 kD
- ￥1180 - 2800
- Bioss
- bs-5840R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5840R |
| 英文名称 | HEPACAM Rabbit pAb |
| 中文名称 | 肝细胞粘附分子抗体 |
| 英文别名 | GlialCAM; HECAM_HUMAN; HEPACAM; Hepatocyte cell adhesion molecule; Protein hepaCAM. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HEPACAM |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Cell adhesion Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cell Adhesion Molecules > Liver |
| 亚基 | Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface. |
| 亚细胞定位 | Cytoplasm. Membrane; Single-pass type I membrane protein; Cytoplasmic side. Note=In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1. |
| 相似性 | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HEPACAM) Polyclonal Antibody, Unconjugated (bs-5840R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 4.88 }}] {Maes, Michaël, et al. "Involvement of connexin43 in acetaminophen-induced liver injury." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2016).} {Other} {="Mouse"}
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HEPACAM Rabbit pAb(bs-5840R)-50ul/100ul/200ul
￥1180 - 2800
