Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-NARF (bs-11173R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
- ￥1180 - 2800
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- bs-11173R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11173R |
| 英文名称 | NARF Rabbit pAb |
| 中文名称 | 核纤层蛋白A识别因子抗体 |
| 英文别名 | DKFZp434G0420; FLJ10067; GC17P078009; IOP2; Iron only hydrogenase like protein 2; Nuclear prelamin A recognition factor; Prenyl dependent prelamin A binding NARF_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NARF |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with LMNA and binds to the farnesylated C-terminal domain. |
| 亚细胞定位 | Nuclear |
| 组织特异性 | Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the NARF family. |
| 功能 | Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Prenylation and methylation are two forms of protein modification, both of which are important for a variety of functions, including membrane attachment, protein-protein interactions and signaling events. NARF (nuclear prelamin A recognition factor), also known as IOP2, is a 456 amino acid nuclear protein that belongs to the NARF family. Expressed ubiquitously with highest expression in heart, skeletal muscle and brain, NARF binds to the C-terminal end of prenylated prelamin A and may be a member of a prelamin A-containing endoprotease complex. Additionally, via its association with prelamin A, NARF may be involved in heterochromatin organization. NARF is expressed as three isoforms due to alternative splicing events and, upon DNA damage, may be phosphorylated by ATM or ATR. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-NARF (bs-11173R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
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NARF Rabbit pAb(bs-11173R)-50ul/100ul/200ul
￥1180 - 2800
