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MARCH6 Rabbit pAb(bs-9340R)-50

ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-9340R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-9340R
    英文名称MARCH6 Rabbit pAb
    中文名称膜相关环指蛋白6抗体
    英文别名Doa10 homolog; E3 ubiquitin protein ligase MARCH6; MARCH 6; MARCH VI; MARCHVI; Membrane associated RING CH protein VI; Membrane associated ring finger(C3HC4) 6; Membrane associated RING finger protein 6; Protein TEB 4; Protein TEB4; RNF 176; RNF176; TEB 4; TEB4; RING finger protein 176; MARH6_HUMAN.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, human lung carcinoma, human lung carcinoma, human lung carcinoma, human lung carcinoma, 肝, 肝, 肝, 肝, Cerebrum, Cerebrum, Cerebrum, Cerebrum, 肾, 肾, 肾, 肾, human kidney carcinoma, human kidney carcinoma, human kidney carcinoma, human kidney carcinoma (Chicken, Dog, Pig, Cow, Horse, Zebrafish, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human MARCH6
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量103 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Interacts with DIO2.
    亚细胞定位Endoplasmic reticulum membrane; Multi pass membrane protein.
    组织特异性Present in brain (at protein level).
    翻译后修饰Auto-ubiquitinated, which results in proteasomal degradation.
    相似性Contains 1 RING-CH-type zinc finger.
    功能E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:50-200}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (bs-9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    ￥1180 - 2800