Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-LRFN4 antibody (bs-11089R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-11089R
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11089R |
| 英文名称 | LRFN4 Rabbit pAb |
| 中文名称 | 富含亮氨酸重复序列/Ⅲ型纤维连接蛋白4抗体 |
| 英文别名 | Alternative namesFIGLER6; leucine rich repeat and fibronectin type III domain containing 4; fibronectin type III, immunoglobulin and leucine rich repeat domains 6; Leucine-rich repeat and fibronectin type-III domain-containing protein 4; Lrfn4; LRFN4_HUMAN; MGC3103; SALM3. |
| 产品应用 | Flow-Cyt=0.2ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LRFN4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 65 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Adhesion Proteins > Membrane Proteins Neuroscience > Neurotransmission > Intracellular Signaling > Adapters |
| 亚基 | Can form heteromeric complexes with LRFN1, LRFN2, LRFN3 and LRFN5. Unable to form homophilic interactions across cell junctions (By similarity). Interacts with DLG1, DLG2, DLG3 and DLG4. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein (By similarity). |
| 翻译后修饰 | Glycosylated (By similarity). |
| 相似性 | Belongs to the LRFN family. Contains 1 fibronectin type-III domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 7 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. |
| 功能 | Promotes neurite outgrowth in hippocampal neurons. May play a role in redistributing DLG4 to the cell periphery. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | LRFN4 is a 635 amino acid single-pass type I membrane protein that belongs to the LRFN family. Containing a fibronectin type-III domain, an Ig-like (immunoglobulin-like) domain, a LRRCT domain, a LRRNT domain and seven LRR (leucine-rich) repeats, LRFN4 is thought to promote neurite outgrowth in hippocampal neurons and may play a role in redistributing PSD-95 to the cell periphery. LRFN4 forms heteromeric complexes with LRFN1, LRFN2, LFRN3 and LFRN5, but does not have the ability to form homomeric complexes across cell junctions of adjacent cells like some other LRFN family members. The PDZ-binding motif of LRFN4 is required for neurite outgrowth promotion and for SAP 97-, NE-dlg- and PSD-95-binding. LRFN4 is encoded by a gene located on human chromosome 11q13.1 and mouse chromosome 19 A. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {0.2ug/test} |
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LRFN4 Rabbit pAb(bs-11089R)-50ul/100ul/200ul
￥1180 - 2800
