Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-KCNE1L antibody (bs-9929R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-9929R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 详细信息
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9929R |
| 英文名称 | KCNE1L Rabbit pAb |
| 中文名称 | 钾离子通道蛋白家族成员1样蛋白抗体 |
| 英文别名 | AMME syndrome candidate gene 2 protein; AMMECR2 protein; Cardiac voltage gated potassium channel accessory subunit 5; KCNE1 like; KCNE5; Mink; MinK like protein; Potassium voltage gated channel subfamily E member 1 like protein; Potassium voltage gated channel, Isk related family, member 1 like; Voltage gated potassium channel accessory subunit 5; KCE1L_HUMAN. |
| 产品应用 | Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human KCNE1L |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 15 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Plasma membrane; Single-pass type I membrane protein. |
| 组织特异性 | Highly expressed in heart, skeletal muscle, brain, spinal cord and placenta. |
| 相似性 | Belongs to the potassium channel KCNE family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | KCNE1L belongs to the potassium channel KCNE family which represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/test} |
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KCNE1L Rabbit pAb(bs-9929R)-50ul/100ul/200ul
￥1180 - 2800
