Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NAP1L1 Polyclonal Antibody, Unconjugated(bs-1052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1052R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 详细信息
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1052R |
| 英文名称 | NAP1L1 Rabbit pAb |
| 中文名称 | 核小体组装蛋白1抗体 |
| 英文别名 | NAP1L; FLJ16112; hNRP; HSP22 like protein interacting protein; MGC23410; MGC8688; NAP 1; NAP 1 related protein; NAP 1L; NAP-1 related protein; NAP-1-related protein; NAP1; NAP1 L1; NAP1 related protein; Nap1l1; NAP1L1 protein; NP1L1_HUMAN; NRP; Nucleosome assembly protein 1 like 1; Nucleosome assembly protein 1-like 1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NAP1L1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Remodeling > INO80 Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis |
| 亚细胞定位 | Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 组织特异性 | Ubiquitously expressed. |
| 翻译后修饰 | Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. |
| 相似性 | Belongs to the nucleosome assembly protein (NAP) family. |
| 功能 | May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. The binding of sequence-specific DNA binding proteins to human nucleosome assembly protein 1 may be an important step contributing to the activation of transcription. May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NAP1L1) Polyclonal Antibody, Unconjugated (bs-1052R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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NAP1L1 Rabbit pAb(bs-1052R)-50ul/100ul/200ul
￥1180 - 2800
