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- bs-8330R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8330R |
| 英文名称 | STK25 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸激酶25抗体 |
| 英文别名 | Serine/threonine kinase 25; Serine/threonine kinase 25(STE20 homolog, yeast); Serine/threonine kinase 25(STE20 homolog, yeast), isoform CRA_a; Serine/threonine kinase 25(yeast); Serine/threonine protein kinase 25; Serine/threonine-protein kinase 25; SOK-1; SOK1; Ste20 like kinase; Ste20 yeast homolog; Ste20-like; STE20-like kinase; Ste20/oxidant stress response kinase 1; Sterile 20(oxidant stress response kinase 1); Sterile 20/oxidant stress-response kinase 1; STK25; STK25_HUMAN; Yeast Sps1/Ste20 related kinase 1; YSK1; C86992. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human STK25/YSK1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway Signal Transduction > Protein Trafficking > Vesicle Transport > Regulation |
| 亚基 | Monomer. |
| 亚细胞定位 | STK25, MST4: Cytoplasmic, Golgi Apparatus. MST3: Cytoplasm. Nucleus. Membrane. Note=The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane. |
| 组织特异性 | MST3: Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. |
| 翻译后修饰 | MST3: Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues. |
| 相似性 | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. |
| 功能 | Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STK25) Polyclonal Antibody, Unconjugated (bs-8330R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-STK25 antibody (bs-8330R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-STK25 antibody (bs-8330R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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STK25 Rabbit pAb(bs-8330R)-50ul/100ul/200ul
￥1180 - 2800
