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- bs-14135R
- 2025年10月16日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14135R |
| 英文名称 | CYC1 Rabbit pAb |
| 中文名称 | 细胞色素C1抗体 |
| 英文别名 | Complex III subunit 4; Complex III subunit IV; CY1_HUMAN; CYC1; Cytochrome b c1 complex subunit 4; Cytochrome b-c1 complex subunit 4; Cytochrome bc1 complex subunit 4; Cytochrome c 1; Cytochrome c-1; Cytochrome c1, heme protein, mitochondrial; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; UQCR4. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Pig, Cow, Monkey) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CYC1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Pathways and Processes > Redox metabolism > Antioxidants Signal Transduction > Metabolism > Mitochondrial Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria |
| 亚细胞定位 | Mitochondrion inner membrane. |
| 翻译后修饰 | Binds 1 heme group per subunit. |
| 相似性 | Belongs to the cytochrome c family. Contains 1 cytochrome c domain. |
| 功能 | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cytochrome c1 is a component of the ubiquinol-cytochrome c reductase complex, which is a respiratory chain that generates an electrochemical potential, coupled to ATP synthesis. Specifically, cytochrome c transfers electrons from the cytochrome bc1 complex to cytochrome c oxidase by transiently binding to the complex. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1). Cytochrome c1 binds one heme per subunit as a result of a mutation-induced collapse of the di-heme cytochrome structure. The cytochrome c1 gene is thought to be regulated by E2F and Sp1 transcription factors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 3.743 }}] {Li Z et al. Zinc oxide nanoparticles induce human multiple myeloma cell death via reactive oxygen species and Cyt-C/Apaf-1/Caspase-9/Caspase-3 signaling pathway in vitro. Biomed Pharmacother. 2020 Feb;122:109712.} {WB} {Human}
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.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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