Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CERS1 Polyclonal Antibody, Unconjugated(bs-5076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-5076R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5076R |
| 英文名称 | Ceramide synthase 1 Rabbit pAb |
| 中文名称 | 长寿相关基因lASS1抗体 |
| 英文别名 | CerS1; LAG1; LASS1; LAG1 homolog ceramide synthase 1; LAG1 longevity assurance homolog 1; LASS 1; Longevity assurance(LAG1 S. cerevisiae) homolog 1; Longevity assurance gene 1; MGC90349; Protein LAG1; UOG 1 protein; UOG1; Upstream of GDF1; Ceramide synthase 1; CERS1_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LASS1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Metabolic disorders Metabolism > Types of disease > Obesity Signal Transduction > Metabolism > Lipid metabolism |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein.
Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Isoform 1 may recycle from the Golgi to the endoplasmic reticulum. |
| 相似性 | Contains 1 TLC (TRAM/LAG1/CLN8) domain. |
| 功能 | May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | LASS1 may be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells it is involved in the production of sphingolipids containing mainly one fatty acid donnor in a fumonisin B1-independent manner. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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Ceramide synthase 1 Rabbit pAb(bs-5076R)-50ul/100ul/200ul
￥1180 - 2800
