Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: THP-1 (Human) Cell Lysate at 30 ug
Lane 3: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-LKB1 (Thr189) (bs-3250R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/48 kD
Observed band size: 48/46 kD
- ￥1180 - 2800
- Bioss
- bs-3250R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3250R |
| 英文名称 | phospho-LKB1 (Thr189) Rabbit pAb |
| 中文名称 | 磷酸化丝氨酸/苏氨酸蛋白激酶抗体 |
| 英文别名 | STK11(phospho T189); LKB1(phospho Thr189); LKB1(phospho T189); p-LKB1(phospho T189); p-LKB1(phospho Thr189); p-LKB1(T189); LKB1; PJS; Renal carcinoma antigen NY-REN-19; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine protein kinase LKB1; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Stk11; STK11_HUMAN; hLKB1; Liver kinase B1; LKB 1; NY REN 19 antigen; Peutz Jeghers syndrome; PJS; Polarization related protein LKB1; Serine/threonine protein kinase STK11; Serine/threonine-protein kinase XEEK1; STK 11. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human LKB1 around the phosphorylation site of Thr189 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Cancer > Cell cycle > Cell cycle inhibitors > p53 pathway Cancer > Oncoproteins/suppressors > Tumor suppressors > p53 pathway Cell Biology > Cell Cycle > Cell Cycle Inhibitors > p53 Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy |
| 亚基 | Catalytic component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with p53/TP53, SMAD4, STK11IP and WDR6. |
| 亚细胞定位 | Nucleus. Cytoplasm. Membrane. Mitochondrion. Note=A small fraction localizes at membranes. Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to mitochondrion during apoptosis. |
| 组织特异性 | Ubiquitously expressed. Strongest expression in testis and fetal liver. |
| 翻译后修饰 | Phosphorylated by ATM at Thr-363 following ionizing radiations (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2. |
| 相似性 | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily. Contains 1 protein kinase domain. |
| 功能 | Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate to transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-LKB1(Thr189) (bs-3250R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-LKB1(Thr189) (bs-3250R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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文献和实验该产品被引用文献
[IF={{ 3.723 }}] {Xiang Li. et al. The novel ginsenoside AD2 prevents angiotensin II-induced connexin 40 and connexin 43 dysregulation by activating AMP kinase signaling in perfused beating rat atria. Chem-Biol Interact. 2021 Mar;:109430} {WB} {Rat}
[IF={{ 3.352 }}] {Zuodong Chen. et al. Oxidative stress induced by hydrogen peroxide promotes glycolysis by activating CaMKK/LKB1/AMPK pathway in broiler breast muscle. Poultry Sci. 2021 Dec;:101681} {WB} {Chicken}
[IF={{ 3.338 }}] {Xu Jiawen. et al. Dietary creatine nitrate enhances muscle creatine loading and delays postmortem glycolysis of broilers that experienced preslaughter transport. J ANIM SCI. 2022 Aug;:} {WB} {Chicken}
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phospho-LKB1 (Thr189) Rabbit pAb(bs-3250R)-50ul/100ul/200ul
￥1180 - 2800
