PDE4D Rabbit pAb(bs-1325R)-50u

l/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-1325R
更新日期2025年10月24日

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应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-1325R
英文名称	PDE4D Rabbit pAb
中文名称	磷酸二酯酶4D抗体
英文别名	cAMP specific 3', 5' cyclic phosphodiesterase 4D; DKFZp686M11213; FLJ97311; cAMP specific 3' 5' cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D; cAMP specific phosphodiesterase PDE4D6; cyclic AMP phosphodiesterase; DPDE 3; DPDE3; Dunce like phosphodiesterase E3; HSPDE4D; PDE 43; PDE43; PDE4DN2; phosphodiesterase 4D cAMP specific(dunce(Drosophila) homolog phosphodiesterase E3); phosphodiesterase 4D cAMP specific(phosphodiesterase E3 dunce homolog Drosophila); phosphodiesterase 4D cAMP specific; phosphodiesterase 4D, cAMP specific(dunce); phosphodiesterase E3 dunce homolog Drosophila; STRK 1; STRK1.
产品应用	WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Mouse, Rat (Human, Dog, Pig, Cow, Horse, Rabbit)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human PDE4D
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	90 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Signal Transduction > Second Messenger > Nucleotide Messenger > cAMP Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR
亚基	Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2.
亚细胞定位	Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
组织特异性	Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
翻译后修饰	Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
相似性	Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
功能	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein protein interactions with cytoskeletal scaffolding proteins.

应用	推荐稀释比例
{WB}	{1:500-2000}
{Flow-Cyt}	{1μg/Test}

Sample:Brain(Mouse) Lysate at 30 ug
Primary: Anti-PDE4D (bs-1325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90kD

Blank control: RSC96(blue), the cells were fixed with 2% P‌‌araformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green).

Sample:Brain(Mouse) Lysate at 30 ug
Primary: Anti-PDE4D (bs-1325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 100 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- PDE4D (bs-1325R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90kD
Observed band size: 90 kD

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