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TRADD Rabbit pAb(bs-1202R)-50u

l/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1202R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1202R
    英文名称TRADD Rabbit pAb
    中文名称肿瘤坏死因子受体1相关死亡域蛋白抗体
    英文别名TNFR1 associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A associated via death domain; TNFRSF1A-associated via death domain; tradd; TRADD_HUMAN; Tumor necrosis factor receptor type 1 associated DEATH domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from the middle of mouse TRADD
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量34 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cell Death > Apoptosis > Receptors > Death receptors & ligands > TRADD

    Cancer > Invasion/microenvironment > Apoptosis > Death receptors & ligands > TRADD

    Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators

    Cell Biology > Apoptosis > Extracellular Signals > Death Ligands

    Cell Biology > Apoptosis > Receptors > Death Receptors

    亚基Interacts with TRIP12. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP.
    亚细胞定位Cytoplasm > cytoskeleton.
    组织特异性Found in all examined tissues.
    相似性Contains 1 death domain.
    功能Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料TNFR1 associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A associated via death domain; TNFRSF1A-associated via death domain; tradd; TRADD_HUMAN; Tumor necrosis factor receptor type 1 associated DEATH domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated(bs-1202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Tissue/cell: rat pancreas tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated(bs-1202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片3
    Sample:
    Lane1: Colon carcinoma (Human)Lysate at 30 ug
    Lane2:Brain(Mouse) Lysate at 30 ug
    Primary: Anti-TRADD (bs-1202R) at 1:200 dilution;
    Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
    Predicted band size : 35kD
    Observed band size : 34kD
    产品细节图片4
    Sample:
    Jurkat(Human) Cell Lysate at 30 ug
    MCF-7(Human) Cell Lysate at 30 ug
    Primary: Anti- TRADD (bs-1202R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 34 kD
    Observed band size: 34 kD
    产品细节图片5
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti- TRADD (bs-1202R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 34 kD
    Observed band size: 34 kD

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    图标文献和实验
    该产品被引用文献

    [IF={{ 2.37 }}] {Chen, Zhiqiang, et al. "Hydrogen sulfide protects against TNF-α induced neuronal cell apoptosis through miR-485-5p/TRADD signaling." Biochemical and Biophysical Research Communications (2016).} {WB} {="Human"}

    相关实验

    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • 白介素1信号通路图

      , inhibitor of NFκ B; IRAK, IL-1R-associated kinase; LTA, lipoteichoic acid; NIK, NFκ B-inducing kinase; PGN, peptidoglycan; RIP, receptor-interacting protein; TNF, tumour necrosis factor; TNFR, TNF receptor; TRADD, TNFR1-associated protein with death domain

    • 亲和层析凝胶指南

      GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin

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    文献支持
    TRADD Rabbit pAb(bs-1202R)-50ul/100ul/200ul
    ￥1180 - 2800