Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-EIF1AY (bs-4314R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 18 kD
- ￥1180 - 2800
- Bioss
- bs-4314R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4314R |
| 英文名称 | EIF1AY Rabbit pAb |
| 中文名称 | 真核翻译起始因子1A抗体 |
| 英文别名 | eIF 1A Y isoform; eIF 4C; eIF-1A Y isoform; EIF-4C; EIF1AY; Eukaryotic translation initiation factor 1A; Eukaryotic translation initiation factor 1A, Y chromosomal; Eukaryotic translation initiation factor 1A, Y linked; Eukaryotic translation initiation factor 4C; IF1AY_HUMAN; Y-chromosomal. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human EIF1AY |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 16 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation |
| 组织特异性 | Ubiquitous. |
| 相似性 | Belongs to the eIF-1A family. Contains 1 S1-like domain. |
| 功能 | Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (bs-4314R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (bs-4314R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验该产品被引用文献
[IF={{ 4.243 }}] {Rongxin Geng. et al. EIF1A depletion restrains human pituitary adenoma progression. Transl Oncol. 2022 Jan;15:101299} {WB,IHC} {Human,Mouse}
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EIF1AY Rabbit pAb(bs-4314R)-50ul/100ul/200ul
￥1180 - 2800
