Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNAJB9 Polyclonal Antibody, Unconjugated(bs-14376R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-14376R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14376R |
| 英文名称 | DNAJB9 Rabbit pAb |
| 中文名称 | DNAJB9蛋白抗体 |
| 英文别名 | DKFZP564F1862; DnaJ(Hsp40) homolog subfamily B member 9; DnaJ homolog subfamily B member 9; DNAJB9; DNJB9_HUMAN; Endoplasmic reticulum DnaJ homolog 4; ERdj4; MDG 1; Mdg-1; Microvascular endothelial differentiation gene 1; Microvascular endothelial differentiation gene 1 protein; MST049; MSTP049; OTTHUMP00000206840. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Chimpanzee) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNAJB9 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins |
| 亚细胞定位 | Cytoplasm. Nucleus. Stress induces its translocation to the nucleus. |
| 相似性 | Contains 1 J domain. |
| 功能 | Acts as a co-chaperone with an Hsp70 protein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. [provided by RefSeq, Dec 2010] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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DNAJB9 Rabbit pAb(bs-14376R)-50ul/100ul/200ul
￥1180 - 2800
