Blank control (Black line):Molt4 (Black).
_x000D_
Primary Antibody (green line): Rabbit Anti-TBR2antibody (bs-11331R)
_x000D_
Dilution: 1μg /10^6 cells;
_x000D_
Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
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Dilution: 1μg /test.
_x000D_
Protocol
_x000D_
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-11331R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11331R |
| 英文名称 | TBR2 Rabbit pAb |
| 中文名称 | 脱中胚蛋白抗体 |
| 英文别名 | Eomes; EOMES_HUMAN; Eomesodermin; Eomesodermin homolog; t box brain 2; T-box brain protein 2; T-brain-2; Tbr 2; TBR-2. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TBR2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 73 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Lineage specification > Trophectoderm Developmental Biology > Reproduction > Germ cell markers Neuroscience > Cell Type Marker > Neuron marker > Soma marker Neuroscience > Neurology process > Neural Signal Transduction Neuroscience > Neurology process > Neurogenesis Stem Cells > Germline Stem Cells > Embryonic Germ Cells Stem Cells > Neural Stem Cells > Intracellular |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in CD8+ T-cells. |
| 相似性 | Contains 1 T-box DNA-binding domain. |
| 功能 | Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | EOMES (eomesodermin homolog), also known as TBR2 (T-box-brain2), is the 686 amino acid human homolog of the mouse Eomes protein that contains one T-box DNA binding domain. Genes that contain T-box domains encode proteins that function as transcription factors and are often involved in the regulation of various developmental events. Localized to the nucleus and expressed in the developing brain, EOMES is thought to be involved in neuronal migration and division and may play a role in trophoblast development and gastrulation. Silencing of the EOMES gene can cause mutated or arrested development and may lead to microcephaly disorders which are characterized by reduced head circumference and a malformed brain. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated(bs-11331R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated(bs-11331R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
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EOMES | TBR2 Rabbit pAb(bs-11331R)-50ul/100ul/200ul
￥1180 - 2800
