AMIGO2 Rabbit pAb(bs-11450R)-50ul/100ul/200ul
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AMIGO2 Rabbit pAb(bs-11450R)-5

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  • ￥1180 - 2800
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  • bs-11450R
  • 2025年10月24日
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-11450R
    英文名称AMIGO2 Rabbit pAb
    中文名称粘附分子IgG样结构域蛋白2抗体
    英文别名Adhesion molecule with Ig like domain 2; ALI 1; ALI1; Alivin 1; Alivin-1; Alivin1; AMIGO 2; Amphoterin induced gene 2; Amphoterin induced protein 2; DEGA; AMGO2_HUMAN; AMIGO-2; Amigo2; Amphoterin induced protein 2; Amphoterin-induced protein 2; Differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; Transmembrane protein AMIGO 2; Transmembrane protein AMIGO2.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow, Horse, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human AMIGO2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量54 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins

    亚基Binds itself as well as AMIGO1 and AMIGO3 (By similarity).
    亚细胞定位Cell membrane; Single-pass type I membrane protein. Nucleus. Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons By
    组织特异性Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
    相似性Belongs to the immunoglobulin superfamily. AMIGO family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    功能A novel primary response gene, AMIGO2, is an activity-dependent gene and promotes survival of neurons. Sequence analyses reveal that rat, mouse, and human AMIGO2 proteins contain seven leucine-rich repeats, one IgC2-like loop and a transmembrane domain, and display homology to Kek and Trk families. Results suggest that expression of ali1 promotes depolarization-dependent survival of the granule neuron. Mouse AMIGO2 was mapped to a locus approximately 55.3 cm from the centromere on chromosome 15 that is syntenic to positional candidate loci for familial Alzheimer's disease type 5 and Parkinson's disease 8 on human chromosome 12.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO-1, AMIGO-2 and AMIGO-3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain, and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. The AMIGO proteins can form complexes with each other, but can also bind itself. AMIGO-1, also designated Alivin-2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO-2 (Alivin-1) is crucial for depolarization-dependent survival. Similar to AMIGO-1 and AMIGO-2, AMIGO-3 (Alivin-3) plays a role in homophilic and/or heterophilic cell-cell interaction and signal transduction.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    AMIGO2 Rabbit pAb(bs-11450R)-5
    Tissue/cell: mouse placenta tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-AMIGO2 Polyclonal Antibody, Unconjugated(bs-11450R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    AMIGO2 Rabbit pAb(bs-11450R)-5
    Sample:
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti- AMIGO2 (bs-11450R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 54 kD
    AMIGO2 Rabbit pAb(bs-11450R)-5
    P‌‌araformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    AMIGO2 Rabbit pAb(bs-11450R)-5
    P‌‌araformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    AMIGO2 Rabbit pAb(bs-11450R)-5
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    [IF={{ 5.923 }}] {Kirsten Hülskötter. et al. Tamoxifen Application Is Associated with Transiently Increased Loss of Hippocampal Neurons following Virus Infection. Int J Mol Sci. 2021 Jan;22(16):8486} {IHC} {Mouse}

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    AMIGO2 Rabbit pAb(bs-11450R)-50ul/100ul/200ul
    ￥1180 - 2800