Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Spleen tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human K562 cell lysates
Lane 9: Human SH-SY5Y cell lysates
Lane 10: Human U-2os cell lysates
Primary: Anti- phospho-FSCN1 (Ser39) (bs-0772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 51 kDa
- ￥1180 - 2800
- Bioss
- bs-0772R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0772R |
| 英文名称 | phospho-FSCN1 (Ser39) Rabbit pAb |
| 中文名称 | 磷酸化纤维束蛋白同源物1抗体 |
| 英文别名 | Fascin(phospho Ser39); Fascin(phospho S39); 55 kDa actin bundling protein; Actin bundling protein; FAN 1; FAN1; Fascin 1; Fascin homolog 1 actin bundling protein(Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; HSN; p55; Singed(Drosophila) like(sea urchin fascin homolog like); Singed drosophila homolog like; Singed like(fascin homolog sea urchin)(Drosophila); Singed like protein; SNL; Strongylocentrotus purpuratus. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins |
| 亚基 | Associates with beta-catenin. Interacts with PLXNB3. |
| 亚细胞定位 | Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium. |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. |
| 相似性 | Belongs to the fascin family. |
| 功能 | Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 4.2 }}] {Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).} {IHC} {="Mouse"}
[IF={{ 3.73 }}] {Majchrzak K, Lo Re D, Gajewska M, Bulkowska M, Homa A, et al. (2013) Migrastatin Analogues Inhibit Canine Mammary Cacer Cell Migrationand Invasion. PLoS ONE 8(10): e76789} {Other} {="Dog"}
[IF={{ 3.31 }}] {Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995.} {Other} {="Dog"}
[IF={{ 0 }}] {Haokang Zhang. et al. The Actin Crosslinker Fascin Regulates Cell Chirality. Advanced Biology. 2023 Jan;:2200240} {WB} {Mouse}
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：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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phospho-FSCN1 (Ser39) Rabbit pAb(bs-0772R)-50ul/100ul/200ul
￥1180 - 2800
