Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-PA26 (bs-8325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
- ￥1180 - 2800
- Bioss
- bs-8325R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8325R |
| 英文名称 | PA26 Rabbit pAb |
| 中文名称 | p53调控PA26核蛋白抗体 |
| 英文别名 | p53 regulated PA26 nuclear protein; p53 regulated protein PA26; p53-regulated protein PA26; sesn1; SESN1_HUMAN; SEST1; sestrin 1; Sestrin-1. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SESN1/PA26 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Metabolism > Types of disease > Obesity |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the sestrin family. |
| 功能 | Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and the mitotic inhibitory protein p21. GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase, and it apparently acts in concert with GADD 153 in inducing growth arrest. A related DNA-damage inducible gene, GADD 34 synergizes with GADD 45 or GADD 153 in suppressing cell growth. PEG-3 (progression elevated gene-3) shares significant homology with GADD 34 and is inducible by DNA damage. An additional GADD related gene, PA26, is a possible target of p53. Three isoforms of PA26 have been identified as PA26-T1, PA26-T2 and PA26-T3. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {3ug/Test} |
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PA26 antibody (bs-8325R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-PA26 antibody (bs-8325R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.14 }}] {Agata Wrońska. et al. Sestrins’ Expression in the Liver Is Not Altered by Short-Term Calorie Restriction in Young and Old Rats. Gerontology. ;:1-9} {WB} {Rat}
[IF={{ 2.5 }}] {Chen-Kai Zhang. et al.Long-term aerobic exercise enhances liver health: miRNA regulation and oxidative stress alleviation..BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2025 Mar 20:759:151677.} {Western blot} {Rat}
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PA26 Rabbit pAb(bs-8325R)-50ul/100ul/200ul
￥1180 - 2800
