Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated(bs-7647R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-7647R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7647R |
| 英文名称 | VDAC3 Rabbit pAb |
| 中文名称 | 线粒体外膜孔蛋白3/电压依赖阴离子通道蛋白3抗体 |
| 英文别名 | hVDAC3; mVDAC3; Outer mitochondrial membrane protein porin 3; voltage dependent anion channel 3; VDAC3_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human VDAC3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 31 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Mitochondrion outer membrane. |
| 组织特异性 | Widely expressed. Highest in testis. |
| 相似性 | Belongs to the eukaryotic mitochondrial porin family. |
| 功能 | Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Large intestine(Rat) Lysate at 40 ug
Primary: Anti- VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Large intestine(Rat) Lysate at 40 ug
Primary: Anti- VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
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文献和实验该产品被引用文献
[IF={{ 6.792 }}] {Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345} {WB} {Human}
[IF={{ 5.638 }}] {Qi H et al. Succinylation-dependent mitochondrial translocation of PKM2 promotes cell survival in response to nutritional stress. Cell Death Dis. 2019 Feb 20;10(3):170.} {CoIP} {Human}
[IF={{ 4.101 }}] {Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11} {WB} {Human}
[IF={{ 3.076 }}] {Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.} {WB} {Mouse}
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VDAC3 Rabbit pAb(bs-7647R)-50ul/100ul/200ul
￥1180 - 2800
