Sample:
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Raw264.7(Mouse) Cell Lysate at 30 ug
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Thymus (Mouse) Lysate at 40 ug
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Primary: Anti- CARD4 (bs-7085R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 108 kD
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Observed band size: 108 kD
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- ￥1180 - 2800
- Bioss
- bs-7085R
- 2026年10月13日
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- 文献和实验
- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7085R |
| 英文名称 | CARD4 Rabbit pAb |
| 中文名称 | 凋亡加强结构域蛋白4抗体 |
| 英文别名 | CARD 4; CARD4; Caspase recruitment domain 4; Caspase recruitment domain containing protein 4; Caspase recruitment domain family member 4; Caspase recruitment domain protein 4; Caspase recruitment domain-containing protein 4; CLR 7.1; CLR7.1; NLR family CARD domain containing 1; NLRC 1; NLRC1; NOD 1; Nod1; NOD1 protein; NOD1_HUMAN; Nucleotide binding oligomerization domain containing 1; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1; Protein Nod1. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CARD4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 108 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Apoptosis > Intracellular > Caspases etc > CARD Family |
| 亚基 | Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. |
| 亚细胞定位 | Cytoplasm. Basolateral cell membrane. Recruited to the basolateral membrane compartment in polarized epithelial cells. |
| 组织特异性 | Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. |
| 相似性 | Contains 1 CARD domain. Contains 9 LRR (leucine-rich) repeats. Contains 1 NACHT domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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NOD1 | CARD4 Rabbit pAb(bs-7085R)-50ul/100ul/200ul
￥1180 - 2800
