Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-Hyaluronidase2 (bs-5888R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
- ￥1180 - 2800
- Bioss
- bs-5888R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5888R |
| 英文名称 | Hyaluronidase2 Rabbit pAb |
| 中文名称 | 透明质酸酶2/玻璃酸酶2抗体 |
| 英文别名 | Hyal 2; Hyal-2; Hyal2; HYAL2_HUMAN; Hyaluronidase 2; Hyaluronidase 2 Precursor; Hyaluronidase-2; Hyaluronoglucosaminidase 2; Hyaluronoglucosaminidase-2; LUCA 2; LUCA-2; LUCA2; Lung carcinoma protein 2; lysosomal hyaluronidase; PH 20 homolog. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HYAL2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > Other Enzymes |
| 亚基 | Interacts with MST1R. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织特异性 | Widely expressed. No expression detected in adult brain. |
| 相似性 | Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. |
| 功能 | Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyluronidase 2 Polyclonal Antibody, Unconjugated(bs-5888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyluronidase 2 Polyclonal Antibody, Unconjugated(bs-5888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验该产品被引用文献
[IF={{ 6.438 }}] {Anna Kocurkova. et al. Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development. BIOFACTORS. 2023 May;:} {IHC} {Mouse}
[IF={{ 4.879 }}] {Petra Žádníková. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251} {WB,IHC,IF} {Human}
[IF={{ 3.6 }}] {Romana Šínová. et al. The hyaluronan metabolism in the UV-irradiated human epidermis and the relevance of in vitro epidermal models. EXP DERMATOL. 2023 Jul;:} {IF} {Human}
[IF={{ 3.1 }}] {Benben Sun. et al. Protecting and rejuvenating ageing skin by regulating endogenous hyaluronan metabolism using adipose-derived stem cell-secreted siRNAs. FRONT MED-LAUSANNE. 2025 Apr;12:} {WB} {Mouse}
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Hyaluronidase2 Rabbit pAb(bs-5888R)-50ul/100ul/200ul
￥1180 - 2800
