Sample:
_x000D_
HepG2(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti- Cortisol Binding Globulin (bs-14001R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 43 kD
_x000D_
Observed band size: 73 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-14001R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14001R |
| 英文名称 | ERPINA6/Cortisol Binding Globulin Rabbit pAb |
| 中文名称 | 皮质醇结合球蛋白抗体 |
| 英文别名 | CBG; CBG_HUMAN; corticosteroid binding globulin; Corticosteroid-binding globulin; serine(or cysteine) proteinase inhibitor clade A(alpha 1 antiproteinase antitrypsin) member 6; serpin A6; serpin peptidase inhibitor clade A(alpha 1 antiproteinase antitrypsin) member 6; SERPINA6; transcortin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cortisol Binding Globulin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells. |
| 翻译后修饰 | N-glycosylated; binds 5 oligosaccharide chains. _x000D_ Glycosylation in position Asn-260 is needed for steroid binding. |
| 相似性 | Belongs to the serpin family. |
| 功能 | Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERPINA6 Polyclonal Antibody, Unconjugated (bs-14001R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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文献和实验该产品被引用文献
[IF=4.4] Guojun Weng. et al. Ag triangle nanoplates assembled on PVC/SEBS membrane as flexible SERS substrates for skin cortisol sensing. SPECTROCHIM ACTA A. 2023 Dec;303:123154 Other
[IF=4.3] Yuan Liang. et al. Withaferin A: A potential selective glucocorticoid receptor modulator with anti-inflammatory effect. FOOD CHEM TOXICOL. 2023 Jul;:113949 WB Human
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SERPINA6 | CBG Rabbit pAb(bs-14001R)-50ul/100ul/200ul
￥1180 - 2800
