Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(bs-10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-10754R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10754R |
| 英文名称 | DNA Ligase III Rabbit pAb |
| 中文名称 | DNA连接酶3抗体 |
| 英文别名 | DNA Ligase 3; D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3_HUMAN; LIG 3; LIG2; LIG3; Ligase II, DNA, ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide synthase [ATP]; Polydeoxyribonucleotide synthase; RP23-316C11.6. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNA Ligase III |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 113 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis > DNA Ligases |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Testis, thymus, prostate and heart. |
| 相似性 | Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain. Contains 1 PARP-type zinc finger. |
| 功能 | Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(bs-10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(bs-10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验相关实验
OGMP - Organelle DNA Library Construction
2 units T7 DNA polymerase 1.5 ul Klenow buffer (10x) --------------------------- in 15 ul final volume. Incubate for 30 min at 12'C. Heat for 5 min to 65'C, let cool down. III. SIZING 1. Separate on LM agarose gel
I. NEBULIZATION of DNA 1. 0.5 - 5 ug DNA in TE (10mM/1mM), 25% glycerol, final volume 500 ul 2. nebulize for 90-100 sec at 5-10 psi (for a medium size of about 1.5kbp) 3. Precipitate with EtOH/Ammonium Acetate
Organelle DNA Library Construction
down for 30 min). Dissolve pellet in 10 ul TE. IV. PHOSPHORYLATION 10 ul sized DNA 2 ul rATP (stock : 10 mM) 2 ul ligase-buffer 10x (similar to kinase buffer) 5 units kinase --------------- in 20 ul
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DNA Ligase III Rabbit pAb(bs-10754R)-50ul/100ul/200ul
￥1180 - 2800
