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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0964R |
| 英文名称 | TRIM32 Rabbit pAb |
| 中文名称 | 神经干细胞抑制相关蛋白TRIM32抗体 |
| 英文别名 | 72 kda Tat interacting Protein; BBS11; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H(autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; Tripartite Motif Containing Protein 32; Zinc Finger Protein HT2A; TRI32_MOUSE. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse TRIM32 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 72 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ubiquitin E3 Enzymes > RING Finger E3 Ligase Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger |
| 亚基 | Interacts with DTNBP1 (By similarity). It self-associates (By similarity). Interacts with PIAS4/PIASY upon treatment with UVB and TNF-alpha. |
| 亚细胞定位 | Cytoplasm. Note=Localized in cytoplasmic bodies, usually concentrated around the nucleus |
| 组织特异性 | Ubiquitous. High expression in brain. |
| 翻译后修饰 | Ubiquitinated. |
| 相似性 | Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 5 NHL repeats. Contains 1 RING-type zinc finger |
| 功能 | Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) (By similarity). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) protein family. TRIM32, like all TRIM proteins, contains a domain structure composed of a B-box, a RING-finger and a coiled-coil motif. Additionally, TRIM32 has six C-terminal NHL domains; it is expressed mainly in the skeletal muscle. The TRIM32 gene encodes an E3 ubiquitin ligase, a protein that attaches ubiquitin to a lysine residue on a target protein and acts in conjunction with ubiquitin-conjugating enzymes UbcH5a, UbcH5c and UbcH6. Mutations in the TRIM32 gene cause two forms of autosomal recessive muscular dystrophy designated limb girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy (STM). TRIM32 mutations can also result in Bardet-Biedl syndrome (BBS), an autosomal recessive disorder characterized by pigmentary retinopathy, polydactyly, hypogenitalism, renal abnormalities, learning disabilities and obesity. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {3ug/Test} |
Primary Antibody (green line): Rabbit Anti-TRIM32 antibody (bs-0964R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.
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文献和实验[IF={{ 4.9 }}] {Jiayu Ning. et al. Inhibitory Effect and Mechanism of the Down-Regulation of TRIM32 in Colorectal Cancer. INT J MOL SCI. 2025 May;26(11):5047} {WB,IF,IHC} {Human}
[IF={{ 3.6 }}] {Lixiang Gu. et al. Mercuric chloride induced brain toxicity in mice: The protective effects of puerarin-loaded PLGA nanoparticles. J BIOCHEM MOL TOXIC. 2023 Jul;:e23425} {IHC,WB} {Mouse}
[IF={{ 2.6 }}] {Fan Jin. et al. Quercetin alleviates kidney damage caused by mercury Chloride: The protective effects of quercetin on autophagy and inflammation were studied based on TRIM32/TLR4/LC3 pathway. TOXICON. 2024 Sep;248:108031} {IHC} {Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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