Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DYNC1I1 Polyclonal Antibody, FITC conjugated(bs-10470R-FITC) 1:200, 60 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
- ￥1180 - 2800
- Bioss
- bs-10470R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10470R |
| 英文名称 | DYNC1I1 Rabbit pAb |
| 中文名称 | 胞浆动力蛋白中间链1抗体 |
| 英文别名 | dynein, cytoplasmic, intermediate polypeptide 1; cytoplasmic dynein 1 intermediate chain 1; DH IC-1; dynein intermediate chain 1, cytosolic; cytoplasmic dynein intermediate chain 1; DC1I1_HUMAN; DYNC1I1; DNCI1; DNCIC1; Dynein cytoplasmic intermediate polypeptide 1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DYNC1I1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 71 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Microtubules Neuroscience > Cell Type Marker > Neuron marker > Axon marker Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Motor Proteins > Dynein |
| 亚基 | Consists of at least two heavy chains and a number of intermediate and light chains. |
| 亚细胞定位 | Cytoplasm, cytoskeleton, cilium axoneme. |
| 相似性 | Belongs to the dynein intermediate chain family.
Contains 5 WD repeats. |
| 功能 | Part of the dynein complex of respiratory cilia. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Blank control(blue): Hela(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti- DYNC1I1/FITC Conjugated antibody (bs-10470R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Primary Antibody: Rabbit Anti- DYNC1I1/FITC Conjugated antibody (bs-10470R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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DYNC1I1 Rabbit pAb(bs-10470R)-50ul/100ul/200ul
￥1180 - 2800
