Nova1 Rabbit pAb(bs-11324R)-50ul/100ul/200ul

Nova1 Rabbit pAb(bs-11324R)-50

ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-11324R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-11324R
    英文名称Nova1 Rabbit pAb
    中文名称神经肿瘤Nova1抗原抗体
    英文别名Neuro oncological ventral antigen 1; Neuro-oncological ventral antigen 1; NOVA 1; Nova-1; NOVA1_HUMAN; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA binding protein Nova 1; RNA-binding protein Nova-1; Ventral neuron specific protein 1; Ventral neuron-specific protein 1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Nova1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量52 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Type Marker > Neuron marker > Soma marker

    亚基Interacts with PTBP2; the interaction is direct (By similarity).
    亚细胞定位Nucleus.
    组织特异性Brain.
    相似性Contains 3 KH domains.
    功能May regulate RNA splicing or metabolism in a specific subset of developing neurons.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Nova-1 and Nova-2 are members of a superfamily of protein regulators of RNA metabolism in neurons. Both are nuclear RNA binding proteins with K homology motifs, conserved protein sequences which bind to RNA (1,2). Nova proteins, normally sequestered in the central nervous system, are expressed by systemic tumors in patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA) (3,4). Nova-1 is expressed in the hindbrain and ventral spinal cord and Nova-2 is expressed in the neocortex and hippocampus (4). Nova-1 is necessary for regulating neuron-specific alternative splicing of the glycine receptor Alpha2 pre-mRNA (5).

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    Nova1 Rabbit pAb(bs-11324R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (bs-11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Nova1 Rabbit pAb(bs-11324R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (bs-11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Nova1 Rabbit pAb(bs-11324R)-50
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti-Nova1 (bs-11324R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52 kD
    Observed band size: 52 kD
    Nova1 Rabbit pAb(bs-11324R)-50
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Cerebrum (Rat) Lysate at 40 ug
    Primary: Anti-Nova1 (bs-11324R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52 kD
    Observed band size: 52 kD
    Nova1 Rabbit pAb(bs-11324R)-50
    Sample:
    DU145(Human) Cell Lysate at 30 ug
    U87MG(Human) Cell Lysate at 30 ug
    Primary: Anti- Nova1 (bs-11324R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52 kD
    Observed band size: 50 kD

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