Sample:
_x000D_
U-2OS(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti- Synaptotagmin XII (bs-11398R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 47 kD
_x000D_
Observed band size: 48 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-11398R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11398R |
| 英文名称 | Synaptotagmin XII Rabbit pAb |
| 中文名称 | 突触结合蛋白12抗体 |
| 英文别名 | SRG1; Synaptotagmin 12; Synaptotagmin-12; Syt12; SYT12_HUMAN; SytXII. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Synaptotagmin XII |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer. Can also form heterodimers |
| 亚细胞定位 | Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. |
| 相似性 | Belongs to the synaptotagmin family. _x000D_ Contains 2 C2 domains. |
| 功能 | May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. The gene has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Blank control:Hela.
_x000D_ Primary Antibody (green line): Rabbit Anti- Synaptotagmin XII antibody (bs-11398R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti- Synaptotagmin XII antibody (bs-11398R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
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SYT12 | SytXII Rabbit pAb(bs-11398R)-50ul/100ul/200ul
￥1180 - 2800
