Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHFR Polyclonal Antibody, Unconjugated(bs-9058R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-9058R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9058R |
| 英文名称 | DHFR Rabbit pAb |
| 中文名称 | 二氢叶酸还原酶抗体 |
| 英文别名 | DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DHFR |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 21 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Amino Acids |
| 亚基 | Homodimer. |
| 亚细胞定位 | Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. |
| 组织特异性 | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
| 相似性 | Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. |
| 功能 | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:50-200} |
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
细胞 2.大鼠类 BRL 肝细胞 LW-3 肝细胞 BRL 3A 肝细胞 NRK 肾细胞 L-6TG 肌母细胞 3.仓鼠类 BHK 幼仓鼠肾 V79 中国仓鼠肺细胞 CHL 中国仓鼠肺细胞 CHO 中国仓鼠卵巢细胞 R1610 中国仓鼠体细胞 *CHO/dhFr- 二氢叶酸还原酶缺陷型CHO 4．人类 2BS 胚肺 XJH B淋巴细胞 HLF 胚肺 L-02 肝细胞
NRK 肾细胞L-6TG 肌母细胞 3.仓鼠类BHK 幼仓鼠肾V79 中国仓鼠肺细胞CHL 中国仓鼠肺细胞CHO 中国仓鼠卵巢细胞R1610 中国仓鼠体细胞*CHO/dhFr- 二氢叶酸还原酶缺陷型CHO4．人类 2BS 胚肺 XJH B淋巴细胞 HLF 胚肺 L-02 肝细胞 WI-38 胚肺 Chang Liver 张氏肝 SL-7 胚肺 QSG-7701 肝细胞 H.A. 羊膜 293 Ad5DNA转化的胚肾 WISH 羊膜
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DHFR Rabbit pAb(bs-9058R)-50ul/100ul/200ul
￥1180 - 2800
