Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated(bs-2663R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-2663R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2663R |
| 英文名称 | CLEC5A Rabbit pAb |
| 中文名称 | C型凝集素结构域家族5成员A抗体 |
| 英文别名 | A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; C-type lectin domain family 5 member A; C type lectin domain family 5 member A; C-type lectin superfamily member 5; CLEC5 A; CLEC5A; CLECSF 5; CLECSF5; MDL 1; MDL-1; MDL1; CLC5A_MOUSE; Myeloid DAP12-associating lectin 1; Myeloid DAP12 associating lectin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse CLEC5A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Immune System Diseases > Antiviral Signaling Immunology > Innate Immunity > Macrophage / Inflamm. Microbiology > Interspecies Interaction > Host Virus Interaction |
| 亚基 | Monomer. Homodimer. The majority of CLEC5A is expressed as a monomeric form on macrophages. Interacts with TYROBP/DAP12. The interaction with TYROBP is required for CLEC5 cell surface expression. Interacts with HCST/DAP10. Forms an CLEC5A/TYROBP/HCST trimolecular complex depending almost solely on TYROBP. |
| 亚细胞定位 | Cell membrane; Single-pass type II membrane protein. |
| 组织特异性 | Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U937 and MonoMac6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells. |
| 翻译后修饰 | N-glycosylated. Contains sialic acid residues. |
| 相似性 | Contains 1 C-type lectin domain. |
| 功能 | Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through phosphylation of TYROBP, this interaction does not result in viral entry but stimulates proinflammatory cytokine release. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CLEC5A(bs-2663R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Primary: Anti-CLEC5A(bs-2663R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLEC5A) Polyclonal Antibody, Unconjugated (bs-2663R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Rat Spinal cord tissue lysates
Primary: Anti-CLEC5A (bs-2663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 37 kDa
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Rat Spinal cord tissue lysates
Primary: Anti-CLEC5A (bs-2663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 37 kDa
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文献和实验该产品被引用文献
[IF={{ 7.7 }}] {Daoxi Qi. et al.Epigenetic upregulation of CLEC5A contributes to monocyte/macrophage dysfunction in coronary artery disease..International Journal of Biological Macromolecules.2025 Mar 25;308(Pt 2):142471.} {IF} {Mouse}
[IF={{ 4.681 }}] {Tong L et al. CLEC5A expressed on myeloid cells as a M2 biomarker relates to immunosuppression and decreased survival in patients with glioma. Cancer Gene Ther. 2019 Oct 8.} {IHC,IF,WB} {Human}
[IF={{ 2 }}] {Wang Xin. et al. REEP5 mediates the function of CLEC5A to alleviate myocardial infarction by inhibiting endoplasmic reticulum stress-induced apoptosis. BMC CARDIOVASC DISOR. 2024 Dec;24(1):1-13} {WB} {Human}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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CLEC5A Rabbit pAb(bs-2663R)-50ul/100ul/200ul
￥1180 - 2800
