Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-0448R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0448R |
| 英文名称 | NIS Rabbit pAb |
| 中文名称 | 钠碘转运体蛋白抗体 |
| 英文别名 | Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I-cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; SLC5A5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5(sodium iodide symporter) member 5; Solute carrier family 5; Solute carrier family 5 member 5. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NIS |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 68 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Signal Transduction > Metabolism > Plasma Membrane > Channels Signal Transduction > Metabolism > Vitamins / Minerals |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. |
| 相似性 | Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. |
| 功能 | Mediates iodide uptake in the thyroid gland. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation.Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Sample:
_x000D_ Lane 1: Mouse Stomach tissue lysates
_x000D_ Lane 2: Rat Stomach tissue lysates
_x000D_ Primary: Anti-NIS (bs-0448R) at 1/200 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 68 kDa
_x000D_ Observed band size: 48 kDa
_x000D_ Lane 1: Mouse Stomach tissue lysates
_x000D_ Lane 2: Rat Stomach tissue lysates
_x000D_ Primary: Anti-NIS (bs-0448R) at 1/200 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 68 kDa
_x000D_ Observed band size: 48 kDa
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SLC5A5 | NIS Rabbit pAb(bs-0448R)-50ul/100ul/200ul
￥1180 - 2800
