TPM2 | Tropomyosin-2 Rabbit pAb(bs-1243R)-50ul/100ul/200ul产品图
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TPM2 | Tropomyosin-2 Rabbit pA

b(bs-1243R)-50ul/100ul/200ul
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  • bs-1243R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1243R
    英文名称TPM2 Rabbit pAb
    中文名称原肌球蛋白抗体
    英文别名AMCD1; beta tropomyosin muscle; BETA-TM; DA1; DA2B; EPITHELIAL TROPOMYOSIN; FIBROBLAST MUSCLE TYPE TROPOMYOSIN; MGC109519; NEM4; TM2; TMSB; TPM2; TROP-2; TROPOMYOSIN 2; Tropomyosin 2(beta chain); TPM2_MOUSE; TPM2_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Chicken, Dog, Cow, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human TPM2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量33 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Tropomyosin

    亚基Heterodimer of an alpha and a beta chain.
    亚细胞定位Cytoplasm, cytoskeleton.
    组织特异性Present in primary breast cancer tissue, absent from normal breast tissue.
    翻译后修饰Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.
    相似性Belongs to the tropomyosin family.
    功能Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: human breast carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-TPM2 Polyclonal Antibody, Unconjugated(bs-1243R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Tissue/cell: mouse stomach wall; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-TPM2 Polyclonal Antibody, Unconjugated(bs-1243R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Sample:
    _x000D_ Small intestine (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti- TPM2 (bs-1243R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 33 kD
    _x000D_ Observed band size: 35 kD
    _x000D_
    产品细节图片4
    Sample:
    _x000D_ Small intestine (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti- TPM2 (bs-1243R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 33 kD
    _x000D_ Observed band size: 35 kD
    _x000D_

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    [IF=4.1] Seunghoon Kim. et al. Multiregional single-cell transcriptomics reveals an association between partial EMT and immunosuppressive states in oral squamous cell carcinoma. ISCIENCE. 2025 Jun IF Human

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    TPM2 | Tropomyosin-2 Rabbit pAb(bs-1243R)-50ul/100ul/200ul
    ￥1180 - 2800