Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated(bs-12364R) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-12364R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12364R |
| 英文名称 | SCXA Rabbit pAb |
| 中文名称 | 碱性螺旋-环-螺旋转录因子SCXA抗体 |
| 英文别名 | Basic helix loop helix transcription factor scleraxis; Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; scleraxis homolog A; SCX; SCX_HUMAN; SCXB. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Chicken, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SCXA |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Efficient DNA binding requires dimerization with another bHLH protein. Dimerizes and binds the E-box consensus sequence with E12 (By similarity). |
| 亚细胞定位 | Nucleus. |
| 相似性 | Contains 1 basic helix-loop-helix (bHLH) domain. |
| 功能 | Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Transcription factors are proteins that bind DNA adjacent to genes and control the production of mRNA transcripts. Scleraxis (basic helix-loop-helix transcription factor scleraxis) is a 201 amino acid protein that dimerizes with another bHLH protein to initiate transcription. Scleraxis is known to play a role in formation of mesoderm and somite-derived chondrogenic lineages. Scleraxis localizes to the nucleus and contains 1 bHLH domain. bHLH transcription factors, in general, function in cellular differentiation, proliferation, and oncogene regulation. The gene encoding Scleraxis maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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SCX | BHLHA41 Rabbit pAb(bs-12364R)-50ul/100ul/200ul
￥1180 - 2800
