CARM1 Rabbit pAb(bs-4098R)-50ul/100ul/200ul
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CARM1 Rabbit pAb(bs-4098R)-50u

l/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-4098R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-4098R
    英文名称CARM1 Rabbit pAb
    中文名称蛋白精氨酸N甲基4抗体
    英文别名CARM1_HUMAN; Coactivator associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4; PRMT4; PRMT 4; Coactivator-associated arginine methyltransferase 1; Histone arginine methyltransferase CARM 1; Histone-arginine methyltransferase CARM1; Protein arginine methyltransferase; Protein arginine N methyltransferase 4.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human CARM1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量66 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins

    Cancer > Oncoproteins/suppressors > Tumor suppressors > p53 pathway

    Cell Biology > Cell Cycle > Cell Cycle Inhibitors > p53

    Epigenetics and Nuclear Signaling > Cell cycle > Cell Cycle Inhibitors > p53

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation > Arginine Methylation

    Epigenetics and Nuclear Signaling > Transcription > Co-factors

    Epigenetics and Nuclear Signaling > Transcription > Other factors

    Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Co-factors

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism

    Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors

    亚基Homodimer (Probable). Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, TRIM24, CREBBP and CTNNB1. Identified in a complex containing CARM1, TRIM24 and NCOA2/GRIP1. Interacts with NCOA3/SRC3. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1.
    亚细胞定位Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
    组织特异性Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
    翻译后修饰Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity).
    Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
    相似性Belongs to the protein arginine N-methyltransferase family.
    功能Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. At least two transcript variants encoding the same protein have been found for this gene (from EntrezGene).

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    CARM1 Rabbit pAb(bs-4098R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CARM1) Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    CARM1 Rabbit pAb(bs-4098R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CARM1) Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    CARM1 Rabbit pAb(bs-4098R)-50u
    Sample:
    NIH/3T3(Mouse) Cell Lysate at 30 ug
    Primary: Anti- CARM1 (bs-4098R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 66 kD
    Observed band size: 66 kD
    CARM1 Rabbit pAb(bs-4098R)-50u
    Sample:
    NIH/3T3(Mouse) Cell Lysate at 30 ug
    DU145(Human) Cell Lysate at 30 ug
    Primary: Anti- CARM1 (bs-4098R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 66 kD
    Observed band size: 66/64 kD
    CARM1 Rabbit pAb(bs-4098R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CARM1) Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    [IF={{ 5.62 }}] {Yun Zhao. et al. Exercise pretreatment alleviates neuroinflammation and oxidative stress by TFEB-mediated autophagic flux in mice with ischemic stroke. EXP NEUROL. 2023 Mar;:114380} {WB} {Mouse}

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    CARM1 Rabbit pAb(bs-4098R)-50ul/100ul/200ul
    ￥1180 - 2800