Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human K562 cell lysates
Lane 4: Human HL-60 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human HUVEC cell lysates
Lane 8: Human A549 cell lysates
Primary: Anti-CBX4 (bs-17376R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
- ￥1180 - 2800
- Bioss
- bs-17376R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-17376R |
| 英文名称 | CBX4 Rabbit pAb |
| 中文名称 | 染色盒同源物4抗体 |
| 英文别名 | CBX 4; CBX4_HUMAN; E3 SUMO-protein ligase CBX4; EC:2.3.2.-; Chromobox protein homolog 4; Polycomb 2 homolog(Pc2; hPc2); Pc2; hPc2; |
| 产品应用 | WB=1:500-2000, ICC/IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CBX4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 61 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > Methylated DNA Epigenetics and Nuclear Signaling > Chromatin Remodeling > Polycomb Silencing Epigenetics and Nuclear Signaling > Chromatin Remodeling > Polycomb Silencing > PRC2 Epigenetics and Nuclear Signaling > Ubiquitin & Ubiquitin Like Modifiers > Ubiquitin like Modifiers |
| 亚基 | Interacts with histone H3-K9Me3. Interacts with CHTOP (By similarity). Component of a PRC1-like complex. Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494. |
| 相似性 | Contains 1 chromo domain. |
| 功能 | E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables SUMO binding activity; SUMO ligase activity; and enzyme binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in nuclear body. Part of PRC1 complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:100-500} |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (bs-17376R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 4.658 }}] {Hu C et al. CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. J Cell Mol Med. 2019 Nov 13.} {WB,IHC} {Human&Mouse}
[IF={{ 4.011 }}] {Xu J et al. Regulatory effect of two Trichinella spiralis serine protease inhibitors on the host's immune system. Sci Rep. 2019 Nov 19;9(1):17045.} {WB} {Mouse}
[IF={{ 1.91 }}] {Han, Xiao, et al. "HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells." Graefe's Archive for Clinical and Experimental Ophthalmology 253.8 (2015): 1279-1290.} {WB} {="Human"}
技术资料暂无技术资料 索取技术资料
文献支持
CBX4 Rabbit pAb(bs-17376R)-50ul/100ul/200ul
￥1180 - 2800
