Blank control: Hela.
_x000D_
Primary Antibody (green line): Rabbit Anti-Dlx1 antibody (bs-6467R)
_x000D_
Dilution: 1μg /10^6 cells;
_x000D_
Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_
Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_
Dilution: 1μg /test.
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Protocol
_x000D_
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-6467R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6467R |
| 英文名称 | Dlx1 Rabbit pAb |
| 中文名称 | 同源转录因子DLX1抗体 |
| 英文别名 | DII B; Distal less homeo box 1; Distal less homeobox 1; Distal-less homeobox gene 1a; Dlx 1; Dlx; Dlx1; Dlx1 distal-less homeobox 1; DLX1_HUMAN; Homeo box protein DLX1; Homeobox protein DLX 1; Homeobox protein DLX-1; Homeobox protein Dlx1a; Homeodomain transcription factor DLX1; RP23-419H3.3. |
| 产品应用 | Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Dlx1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families Neuroscience > Neurology process > Neurogenesis Stem Cells > Hematopoietic Progenitors > Intracellular Molecules |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the distal-less homeobox family. Contains 1 homeobox DNA-binding domain. |
| 功能 | Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/test} |
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Dlx1 Rabbit pAb(bs-6467R)-50ul/100ul/200ul
￥1180 - 2800
