Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0075R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0075R |
| 英文名称 | Nociceptin Rabbit pAb |
| 中文名称 | 孤菲肽/痛敏肽抗体 |
| 英文别名 | PNOC; N/OFQ; N23K; N23K/N27K; Nociceptin, included; Npnc1; OFQ; OFQ/N; Orphanin FQ; PPNOC; prepronociceptin; Propronociceptin; PNOC_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Nociceptin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmitter > Neuropeptides > Opioids Neuroscience > Sensory System > Somatosensory system > Nociception |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Predominantly in the brain and spinal cord. |
| 相似性 | Belongs to the opioid neuropeptide precursor family. |
| 功能 | Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Nociceptin/orphanin FQ (N/OFQ), a 17-amino acid neuropeptide, is the endogenous ligand for the nociceptin receptor (NOP, ORL-1), and initiates its function to act on numerous brain activities such as pain sensation and fear learning. It is derived from the prepronociceptin protein, as are a further 2 peptides, nocistatin & NocII, which inhibit the N/OFQ receptor function. Nociceptin itself acts as a potent anti-analgesic, effectively counteracting the effect of pain-relievers. The gene coding for prepronociceptin is located on Ch8p21 in humans. Nociceptin acts at the Nociceptin receptor (NOP receptor) formerly known as ORL1. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Brain(Rat) lysate at 30ug;
_x000D_ Liver(Rat) lysate at 30ug;
_x000D_ Primary: Anti-Nociceptin(bs-0075R) at 1:200 dilution;
_x000D_ Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
_x000D_ Predicted band size : 20kD
_x000D_ Observed band size : 20kD
_x000D_ _x000D_
_x000D_ Brain(Rat) lysate at 30ug;
_x000D_ Liver(Rat) lysate at 30ug;
_x000D_ Primary: Anti-Nociceptin(bs-0075R) at 1:200 dilution;
_x000D_ Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
_x000D_ Predicted band size : 20kD
_x000D_ Observed band size : 20kD
_x000D_ _x000D_
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文献和实验该产品被引用文献
[IF=1.931] Lyu J et al. Botulinum toxin A alleviates orofacial nociception induced by orthodontic tooth movement through nociceptin/orphanin-FQ pathway in rats. Arch Oral Biol . 2020 Sep;117:104817. WB Rat
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PNOC | Nociceptin Rabbit pAb(bs-0075R)-50ul/100ul/200ul
￥1180 - 2800
