Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Midnolin Polyclonal Antibody, Unconjugated(bs-0761R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-0761R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0761R |
| 英文名称 | Midnolin Rabbit pAb |
| 中文名称 | 中脑核仁蛋白抗体 |
| 英文别名 | MIDN_HUMAN; 3000003C15Rik; DKFZp547M072; MIDN; midnolin; Midbrain nucleolar protein. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, GuineaPig, Danio) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MIDN |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 亚细胞定位 | Nucleus, nucleolus. |
| 相似性 | Contains 1 ubiquitin-like domain. |
| 功能 | May be involved in regulation of genes related to neurogenesis in the nucleolus. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 该蛋白可能参与中脑神经核仁的相关调节。 |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-Midnolin (bs-0761R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-Midnolin (bs-0761R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Midnolin Rabbit pAb(bs-0761R)-50ul/100ul/200ul
￥1180 - 2800
