Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MRCK alpha Polyclonal Antibody, Unconjugated(bs-10952R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-10952R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10952R |
| 英文名称 | MRCK alpha Rabbit pAb |
| 中文名称 | CDC42结合蛋白激酶α/MRCKα抗体 |
| 英文别名 | CDC42BPA; CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha(DMPK like); CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha dmpk like; CDC42 binding protein kinase beta; CDC42-binding protein kinase alpha; DKFZp686L1738; DMPK like; DMPK-like alpha; FLJ23347; KIAA0451; mrck; mrcka; MRCKA_HUMAN; Myotonic dystrophy kinase related CDC42 binding protein kinase alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; Myotonic dystrophy protein kinase-like alpha; pk428; RP5-1087E8.4; Ser thr protein kinase PK428; Ser thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine protein kinase MRCK alpha; Serine/threonine-protein kinase MRCK alpha; DKFZp686P1738; MGC189823; A930014J19Rik. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MRCK alpha |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 197 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPA and MYO18A. LURAP1 binding results in activation of CDC42BPA by abolition of its negative autoregulation. |
| 亚细胞定位 | Cytoplasm. Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. |
| 组织特异性 | Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. |
| 功能 | May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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MRCK alpha Rabbit pAb(bs-10952R)-50ul/100ul/200ul
￥1180 - 2800
