Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSP40 Polyclonal Antibody, Unconjugated(bs-6002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-6002R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6002R |
| 英文名称 | HSP40 Rabbit pAb |
| 中文名称 | 热休克蛋白家族40抗体 |
| 英文别名 | HSP40 homolog; DnaJ(Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNAJB4/HSP40 homolog |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins |
| 亚基 | Homodimer. |
| 亚细胞定位 | Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. |
| 组织特异性 | Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver. |
| 相似性 | Contains 1 J domain. |
| 功能 | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | DNAJB4 is a member of the evolutionarily conserved DNAJ/HSP40 protein family. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2μg/Test} |
This image has been kindly submitted by Tübingen Ageing and Tumour Immunology Group (TATI), Centre for Medical Research (ZMF), University of Tübingen Medical School www.tati-group.de via our distributor Biozol.Human Melanoma cell line, EST02, was stained with Rabbit Anti-HSP40 homolog Polyclonal Antibody, FITC Conjugated (bs-6002-FITC) at 1:50 for 30 minutes, on ice.
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP40) Polyclonal Antibody, Unconjugated (bs-6002R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
关于论坛中所有Native PAGE/非变性电泳及蛋白回收的经典总结
5倍体积的浸提液，4 ℃过夜，期间振摇3～4次。5. 第二天，4 ℃，10000 r/m离心10 min，留取上清。6. 使用透析袋，在相应的缓冲液中，4 ℃透析24—48小时7. 进行SDS-PAGE电泳鉴定和蛋白含量测定。注意事项：（切记！）回收前蛋白若为包涵体形式，回收后透析时透析液应加相应变性剂，以免沉淀。将胶在1mM DTT,20mM MgCl2种浸泡4h或过夜，将目的条带切下，置透析带中，电泳，然后收集上清液即为目的蛋白。Native PAGENative PAGE 分离碱性蛋白，要用
技术资料暂无技术资料 索取技术资料
HSP40 Rabbit pAb(bs-6002R)-50ul/100ul/200ul
￥1180 - 2800
