Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-ETS1 Polyclonal Antibody, Unconjugated(bs-1285R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-1285R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1285R |
| 英文名称 | ETS1 Rabbit pAb |
| 中文名称 | 转录因子家族ETS1抗体 |
| 英文别名 | Avian erythroblastosis virus E26(v ets) oncogene homolog 1; C ets 1 protein; ETS 1; Ets protein; ETS-1; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; p54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Ets1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors Cardiovascular > Heart > Cardiogenesis > Transcription factors/regulators Epigenetics and Nuclear Signaling > Transcription > Domain Families > Ets Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚基 | Interacts with MAF and MAFB. Interacts with PAX5, the interaction alters DNA-binding properties. Binds to DAXX. Interacts with UBE2I. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity. _x000D_ Ubiquitinated; which induces proteasomal degradation. |
| 相似性 | Belongs to the ETS family. _x000D_ Contains 1 ETS DNA-binding domain. _x000D_ Contains 1 PNT (pointed) domain. |
| 功能 | Transcription factor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Sample:Spleen (Mouse) Lysate at 30 ug
_x000D_ Primary: Anti- ETS1 (bs-1285R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
_x000D_ Predicted band size: 49 kD
_x000D_ Observed band size: 49 kD
_x000D_
_x000D_ Primary: Anti- ETS1 (bs-1285R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
_x000D_ Predicted band size: 49 kD
_x000D_ Observed band size: 49 kD
_x000D_
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ETS1 antibody (bs-1285R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-ETS1 antibody (bs-1285R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
_x000D_ Lane 1: Mouse Spleen tissue lysates
_x000D_ Lane 2: Mouse Thymus tissue lysates
_x000D_ Lane 3: Mouse Lung tissue lysates
_x000D_ Lane 4: Rat Lymph node tissue lysates
_x000D_ Lane 5: Human Jurkat cell lysates
_x000D_ Lane 6: Human U251 cell lysates
_x000D_ Primary: Anti-ETS1 (bs-1285R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 49 kDa
_x000D_ Observed band size: 50 kDa
_x000D_ Lane 1: Mouse Spleen tissue lysates
_x000D_ Lane 2: Mouse Thymus tissue lysates
_x000D_ Lane 3: Mouse Lung tissue lysates
_x000D_ Lane 4: Rat Lymph node tissue lysates
_x000D_ Lane 5: Human Jurkat cell lysates
_x000D_ Lane 6: Human U251 cell lysates
_x000D_ Primary: Anti-ETS1 (bs-1285R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 49 kDa
_x000D_ Observed band size: 50 kDa
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文献和实验该产品被引用文献
[IF=2.4] Shu Li. et al.Unraveling the Dual Role of circ-CBLB and ETS-1 in Rheumatoid Arthritis: Biomarkers and Therapeutic Targets..International Journal of Rheumatic Diseases.2025 Mar;28(3):e70167. Cck-8 assay Human
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ETS1 Rabbit pAb(bs-1285R)-50ul/100ul/200ul
￥1180 - 2800
