PROC | Protein C Rabbit pAb(bs-0040R)-50ul/100ul/200ul产品图

PROC | Protein C Rabbit pAb(bs

-0040R)-50ul/100ul/200ul
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  • bs-0040R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0040R
    英文名称protein C Rabbit pAb
    中文名称维生素K依赖的蛋白C重链抗体
    英文别名Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; EC 3.4.21.69; PC; PROC; PROC1; Vitamin K dependent protein C precursor; APC; EC 3.4.21.69; PC; proC; PROC_HUMAN; Protein C(inactivator of coagulation factors Va and VIIIa); Vitamin K dependent protein C; Vitamin K-dependent protein C; Anticoagulant protein C; Vitamin K-dependent protein C heavy chain.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Vitamin K-dependent protein C heavy chain
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量29/46 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.
    组织特异性Plasma; synthesized in the liver.
    翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium._x000D_
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans._x000D_
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains._x000D_
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
    相似性Belongs to the peptidase S1 family._x000D_
    Contains 2 EGF-like domains._x000D_
    Contains 1 Gla (gamma-carboxy-glutamate) domain._x000D_
    Contains 1 peptidase S1 domain.
    功能Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat colitis tissue;4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-Activated protein C/PROC1 Polyclonal Antibody, Unconjugated(bs-0040R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
    _x000D_
    产品细节图片2
    Tissue/cell: Human brain glioma tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-Activated protein C/PROC1 Polyclonal Antibody, Unconjugated(bs-0040R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Tissue/cell: Rat liver tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-protein C Polyclonal Antibody, Unconjugated(bs-0040R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片4
    Tissue/cell: Mouse spleen tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-protein C Polyclonal Antibody, Unconjugated(bs-0040R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片5
    Sample:
    _x000D_ Lane 1: Rat Kidney tissue lysates
    _x000D_ Lane 2: Rat Colon tissue lysates
    _x000D_ Lane 3: Rat H9C2 cell lysates
    _x000D_ Primary: Anti-protein C (bs-0040R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 29/46 kDa
    _x000D_ Observed band size: 52 kDa

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