Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated(bs-2123R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-2123R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2123R |
| 英文名称 | Cyp2J3 Rabbit pAb |
| 中文名称 | 细胞色素P450Ⅱj3抗体 |
| 英文别名 | CP2J3_RAT; cytochrome P450, family 2, subfamily j, polypeptide 3; cytochrome P450 monooxygenase; Cyp2j9; cytochrome P450, family 2, subfamily j, polypeptide 9; cytochrome P450, family 2, subfamily j, polypeptide 3; CYPIIJ3; cytochrome P450 2J3. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from rat Cyp2-j3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| 组织特异性 | Abundantly expressed in heart and liver. |
| 相似性 | Belongs to the cytochrome P450 family. |
| 功能 | This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation mainly to 14,15-, 11,12-, and 8,9-epoxyeicosatrienoic acids (EET). It also acts as an omega-1-hydroxylase by metabolizing arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-OH-AA). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins and mutagens. They can be divided into 4 classes, according to the method by which electrons from NAD(P)H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography and structural fold are highly conserved. The conserved core is composed of a coil termed the 'meander', a four-helix bundle, helices J and K, and two sets of beta-sheets. These constitute the haem-binding loop (with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron), the proton-transfer groove and the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins, most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyse regiospecific and stereospecific oxidation of non-activated hydrocarbons at physiological temperatures. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 5.5 }}] {Huang X et al. Ophiopogonin D Reduces Myocardial Ischemia-Reperfusion Injury via Upregulating CYP2J3/EETs in Rats.(2018) Cellular Physiology and Biochemistry.49(4):1646-1658..} {WB} {Rat}
[IF={{ 5.076 }}] {Wang J et al. Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 UpregulationOxid Med Cell Longev.2020 Dec 31;2020:8857906.} {WB} {Rat、Human}
[IF={{ 3.457 }}] {Zhang J et al. EETs/PPARs activation together mediates the preventive effect of naringenin in high glucose-induced cardiomyocyte hypertrophy.(2019)Biomed Pharmacother;Jan;109:1498-1505.} {WB} {}
[IF={{ 3.22 }}] {You et al. Ophiopogonin D maintains Ca2+ homeostasis in rat cardiomyocytes in vitro by upregulating CYP2J3/EETs and suppressing ER stress. (2016) Acta.Pharmacol.Sin. 37:368-81} {WB} {Rat}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450
from microsomes through the use of proteases were not successful, resulting merely in the solubilization of a number of other microsomal proteins. The breakthrough event was the solubrlizatron of rabbit liver microsomal cytochrome P450 using the ionic detergent
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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Cyp2J3 Rabbit pAb(bs-2123R)-50ul/100ul/200ul
￥1180 - 2800
