Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Insig1 (bs-5074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
- ￥1180 - 2800
- Bioss
- bs-5074R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5074R |
| 英文名称 | Insig1 Rabbit pAb |
| 中文名称 | 胰岛素诱导基因1抗体 |
| 英文别名 | CL 6; CL6; INSIG 1; INSIG 1 membrane protein; INSIG1 membrane protein; Insulin induced gene 1; Insulin induced gene 1 protein; insulin induced gene-1; insulin induced gene1; MGC1405; INSI1_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Insig1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 30 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cholesterol Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Cholesterol Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Types of disease > Cancer Signal Transduction > Protein Trafficking > ER Proteins |
| 亚基 | Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with HMGCR (via its SSD); the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with AMFR/gp78 (via its membrane domain); the interaction recruits HMCR at the ER membrane for its ubiquitination and degradation by the sterol-mediated ERAD pathway. |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed in all tissues tested with highest expression in the liver. |
| 翻译后修饰 | Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139. |
| 相似性 | Belongs to the INSIG family. |
| 功能 | Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element binding protein (SREBP) mediated signaling pathway. Insig1 is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. Insig1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. It may also play a role in growth and differentiation of tissues involved in metabolic control, and may play a regulatory role during G0/G1 transition of cell growth. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 5.6 }}] {Lu Zhu. et al. Fatty Acid Desaturation Is Suppressed in Mir-26a/b Knockout Goat Mammary Epithelial Cells by Upregulating INSIG1. INT J MOL SCI. 2023 Jan;24(12):10028} {WB} {Sheep}
[IF={{ 5.168 }}] {Yu et al. LncRNA as ceRNAs may be involved in lactation process. (2017) Oncotarget. 8:98014-98028} {IF} {Goat}
[IF={{ 4.9 }}] {Xingtong Chen. et al.Animal Model Screening for Hyperlipidemic ICR Mice..INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.2025 Feb 27;26(5):2142.} {Western blot} {Mouse}
[IF={{ 4.192 }}] {Huang L et al. CRISPR/Cas9 Based Knockout of miR-145 Affects Intracellular Fatty Acid Metabolism by Targeting INSIG1 in Goat Mammary Epithelial Cells. J Agric Food Chem . 2020 May 6;68(18):5138-5146.} {WB} {goat}
[IF={{ 3.9 }}] {Wang et al. MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting the INSIG1 gene. (2016) RNA.Bio. 13:500-10} {WB} {goat}
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Insig1 Rabbit pAb(bs-5074R)-50ul/100ul/200ul
￥1180 - 2800
